trap activity staining Search Results


trap activity staining  (Beijing Solarbio Science)
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Beijing Solarbio Science trap activity staining
a The <t>TRAP</t> stainning showing that P.histicola inhibit the osteoclast formation on the bone surface in the OVX group (n=3 per group). b-c Quantitative analyses of osteoclast parameters including N.Oc/BS and Oc.S/B (n=4 per group). d-e Peripheral blood level of bone formation maker P1NP and bone resorption marker CTX-1(n=10 per group). f-h Levels of pro-resorptive cytokines RANK, RANKL and OPG expression in tibia among three groups(n=6 per group). i The levels of osteoclastogenesis related inflammatory cytokines in peripheral blood(n=10 per group). j-l Levels of osteoclastogenic cytokines IL-1β, IL-6 and TNFα expression in tibia among three groups(n=6 per group). *** p<0.001, ** p<0.01, * p<0.05. TRAP, tartrate-resistant acid <t>phosphatase;</t> N.Oc/BS, osteoclast number/bone surface; Oc.S/BS, osteoclast surface/bone surface; IL-1β, interluekin-1β; IL-6, interluekin-6; TNFα, tumor necrosis factor α. RANK, receptor activator of nuclear factor κB; RANKL, receptor activator of nuclear factor κB ligand; OPG, osteoprotegerin; P1NP, N-terminal propeptide of type 1 procollagen; CTX-1, cross-linked C-terminal telopeptide of type I collagen; ns, non-significant; P.histicola, Prevotella histicola.
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a The TRAP stainning showing that P.histicola inhibit the osteoclast formation on the bone surface in the OVX group (n=3 per group). b-c Quantitative analyses of osteoclast parameters including N.Oc/BS and Oc.S/B (n=4 per group). d-e Peripheral blood level of bone formation maker P1NP and bone resorption marker CTX-1(n=10 per group). f-h Levels of pro-resorptive cytokines RANK, RANKL and OPG expression in tibia among three groups(n=6 per group). i The levels of osteoclastogenesis related inflammatory cytokines in peripheral blood(n=10 per group). j-l Levels of osteoclastogenic cytokines IL-1β, IL-6 and TNFα expression in tibia among three groups(n=6 per group). *** p<0.001, ** p<0.01, * p<0.05. TRAP, tartrate-resistant acid phosphatase; N.Oc/BS, osteoclast number/bone surface; Oc.S/BS, osteoclast surface/bone surface; IL-1β, interluekin-1β; IL-6, interluekin-6; TNFα, tumor necrosis factor α. RANK, receptor activator of nuclear factor κB; RANKL, receptor activator of nuclear factor κB ligand; OPG, osteoprotegerin; P1NP, N-terminal propeptide of type 1 procollagen; CTX-1, cross-linked C-terminal telopeptide of type I collagen; ns, non-significant; P.histicola, Prevotella histicola.

Journal: bioRxiv

Article Title: An emerging role of Prevotella histicola on estrogen-deficiency induced bone loss through the gut microbiota-bone axis

doi: 10.1101/2020.06.03.133082

Figure Lengend Snippet: a The TRAP stainning showing that P.histicola inhibit the osteoclast formation on the bone surface in the OVX group (n=3 per group). b-c Quantitative analyses of osteoclast parameters including N.Oc/BS and Oc.S/B (n=4 per group). d-e Peripheral blood level of bone formation maker P1NP and bone resorption marker CTX-1(n=10 per group). f-h Levels of pro-resorptive cytokines RANK, RANKL and OPG expression in tibia among three groups(n=6 per group). i The levels of osteoclastogenesis related inflammatory cytokines in peripheral blood(n=10 per group). j-l Levels of osteoclastogenic cytokines IL-1β, IL-6 and TNFα expression in tibia among three groups(n=6 per group). *** p<0.001, ** p<0.01, * p<0.05. TRAP, tartrate-resistant acid phosphatase; N.Oc/BS, osteoclast number/bone surface; Oc.S/BS, osteoclast surface/bone surface; IL-1β, interluekin-1β; IL-6, interluekin-6; TNFα, tumor necrosis factor α. RANK, receptor activator of nuclear factor κB; RANKL, receptor activator of nuclear factor κB ligand; OPG, osteoprotegerin; P1NP, N-terminal propeptide of type 1 procollagen; CTX-1, cross-linked C-terminal telopeptide of type I collagen; ns, non-significant; P.histicola, Prevotella histicola.

Article Snippet: Tartrate-resistant acid phosphatase (TRAP) activity staining were performed according to the manufacturer’s protocol (Solarbio, Beijing, China).

Techniques: Staining, Marker, Expressing